LOCUS       V003133                 8774 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003133
VERSION     V003133
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Brehm J, Salmond G, Minton N.
  TITLE     Sequence of the adenine methylase gene of the Streptococcus faecalis
            plasmid pAM beta 1
  JOURNAL   Nucleic Acids Res. 15 (7), 3177 (1987)
   PUBMED   3104884
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Rose RE.
  TITLE     The nucleotide sequence of pACYC184
  JOURNAL   Nucleic Acids Res. 16 (1), 355 (1988)
   PUBMED   3340533
REFERENCE   3  (bases 1025 to 1263)
  AUTHORS   Kilic AO, Vijayakumar MN, al-Khaldi SF.
  TITLE     Identification and nucleotide sequence analysis of a
            transfer-related region in the streptococcal conjugative transposon
            Tn5252
  JOURNAL   J. Bacteriol. 176 (16), 5145-5150 (1994)
   PUBMED   8051031
REFERENCE   4  (bases 1 to 8774)
  AUTHORS   Provvedi R, Manganelli R, Pozzi G.
  TITLE     Characterization of conjugative transposon Tn5251 of Streptococcus
            pneumoniae
  JOURNAL   FEMS Microbiol. Lett. 135 (2-3), 231-236 (1996)
   PUBMED   8595862
REFERENCE   5  (bases 1 to 8774)
  AUTHORS   Manganelli R, Provvedi R, Berneri C, Oggioni MR, Pozzi G.
  TITLE     Insertion vectors for in vivo construction of recombinant
            conjugative transposons
  JOURNAL   Unpublished
REFERENCE   6  (bases 1 to 8774)
  AUTHORS   Manganelli R, Provvedi R, Berneri C, Oggioni MR, Pozzi G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-1996) Microbiology, University of Siena, Via
            Laterina 8, Siena, Si 53100, Italy
REFERENCE   7  (bases 1 to 8774)
  TITLE     Direct Submission
REFERENCE   8  (bases 1 to 8774)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1987"; volume: "15"; issue: "7"; pages: "3177"
            SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1988"; volume: "16"; issue: "1"; pages: "355"
            SGRef: number: 3; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "1994"; volume: "176"; issue: "16"; pages:
            "5145-5150"
            SGRef: number: 4; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett. 135 (2-3), 231-236 (1996)"
            SGRef: number: 5; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 6; type: "Journal Article"; journalName: "Submitted
            (04-SEP-1996) Microbiology, University of Siena, Via Laterina 8,
            Siena, Si 53100, Italy"
            SGRef: number: 7; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1028
                     /note="similar to Enterococcus faecalis Tn916 of GenBank
                     Accession Number U09422"
                     /citation=[4]
     misc_feature    1025..1263
                     /note="similar to S. pneumoniae Tn5252 of GenBank Accession
                     Number L29324"
                     /citation=[3]
     CDS             complement(1625..2281)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2282..2384)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2910..3455)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3717..4451)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_feature    4459..5370
                     /note="similar to S. agalactiae pIP501 of GenBank Accession
                     Number U00453"
     CDS             5422..6609
                     /label="TcR"
                     /note="tetracycline efflux protein"
     misc_feature    7423..8773
                     /note="similar to S. pneumoniae tet(M) gene of GenBank
                     Accession Number X90939"
                     /citation=[4]
ORIGIN
        1 aacatacttc ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag
       61 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
      121 ggtgtgaagt atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa
      181 tcggttatcc agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga
      241 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa
      301 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc
      361 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
      421 acggtacaag ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt
      481 ggagcttccc cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa
      541 gctggaatta acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca
      601 ctatcggaag cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct
      661 tatattactc atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca
      721 atcggttatg ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga
      781 atcaaacaat gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa
      841 aacagatacc aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg
      901 tgattgcctt gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta
      961 cagctataga tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata
     1021 ctcatcatca ccagtagttc tctctgaatt gtttgtggtt taacagagac acaccaagtc
     1081 tgaaaccaat tttcttttaa ctctccaaat gttgtaatca gttcagggct aaactgactt
     1141 tcaaatgaag tagttagtct atctatctta tcaagaacct ctctttcagc ttgtttcctc
     1201 gccctactag tattcctagt gtaacttaca gttactgatt tccactttcc tgttagtgga
     1261 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc
     1321 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac
     1381 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact
     1441 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc
     1501 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta
     1561 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa
     1621 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1681 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1741 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1801 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1861 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1921 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1981 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2041 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2101 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2161 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2221 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2281 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2341 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     2401 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     2461 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     2521 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     2581 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
     2641 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
     2701 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
     2761 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     2821 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     2881 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     2941 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     3001 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     3061 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     3121 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     3181 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     3241 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     3301 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     3361 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     3421 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     3481 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     3541 tacgatataa gttgtaattc tcatgtttga cagcttatca tcgatacaaa ttccccgtag
     3601 gcgctaggga cctctttagc tccttggaag ctgtcagtag tatacctaat aatttatcta
     3661 cattcccttt agtaacgtgt aactttccaa atttacaaaa gcgactcata gaattatttc
     3721 ctcccgttaa ataatagata actattaaaa atagacaata cttgctcata agtaacggta
     3781 cttaaattgt ttactttggc gtgtttcatt gcttgatgaa actgattttt agtaaacagt
     3841 tgacgatatt ctcgattgac ccattttgaa acaaagtacg tatatagctt ccaatattta
     3901 tctggaacat ctgtggtatg gcgggtaagt tttattaaga cactgtttac ttttggttta
     3961 ggatgaaagc attccgctgg cagcttaagc aattgctgaa tcgagacttg agtgtgcaag
     4021 agcaacccta gtgttcggtg aatatccaag gtacgcttgt agaatccttc ttcaacaatc
     4081 agatagatgt cagacgcatg gctttcaaaa accacttttt taataatttg tgtgcttaaa
     4141 tggtaaggaa tactcccaac aattttatac ctctgtttgt tagggaattg aaactgtaga
     4201 atatcttggt gaattaaagt gacacgagta ttcagtttta atttttctga cgataagttg
     4261 aatagatgac tgtctaattc aatagacgtt acctgtttac ttattttagc cagtttcgtc
     4321 gttaaatgcc ctttacctgt tccaatttcg taaacggtat cggtttcttt taaattcaat
     4381 tgttttatta tttggttgag tactttttca ctcgttaaaa agttttgaga atattttata
     4441 tttttgttca tgtaatcact ccttcttaat tacaaatttt tagcatctaa tttaacttca
     4501 attcctatta tacaaaattt taagatactg cactatcaac acactcttaa gtttgcttct
     4561 aagtcttatt tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat
     4621 gatatggtgc aagtcagcac gaacacgaac cgtcttatct cccattatat ctttttttgc
     4681 actgattggt gtatcatttc gtttttcttt tgtgcgccta aatttcccac aatcactcac
     4741 ttctttctat ttcttcttat tcttatttta tcatcaacaa tcacaaatca cttgtgattt
     4801 gtgataagtg atttgtgatt aatatataaa agccctcttt aaagggcttt tatgtttatt
     4861 ttgagaaaga tataaaatca atatatccct tttctccgat tttacaacgg cattgtagga
     4921 ctttctatct ttcgttttga ttccttttac cagggtttct tttccctcta gtaattcttt
     4981 tacatttgtt ttggtgagtt ttttctttct aaaatgttca gctaaagtaa acttacattc
     5041 aggataattt gaacaaccat aaaacgattt ttttaataca atattgttgc cacacttagg
     5101 acattttcct acaatacttt tttctgcttc tttttctttc tgttcctggt aatcagaaaa
     5161 atttagtttt tctatatcgt taggtacagg actccagtaa atgaacaatg aatttttgat
     5221 attcgtaata aagttctctt gattgccttc tcttttaccg atttttttta aatacgtttc
     5281 ccatttagcc gtcatttcag cactcgttaa aaggtgctga ctttcaactg cctggcacaa
     5341 taattttcct ttttcagtta caacaagctt taatgcggta gtttatcaca gttaaattgc
     5401 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     5461 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     5521 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     5581 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     5641 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     5701 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     5761 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     5821 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     5881 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     5941 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     6001 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     6061 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     6121 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     6181 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     6241 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     6301 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     6361 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     6421 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg
     6481 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     6541 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     6601 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg
     6661 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat
     6721 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct
     6781 ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt
     6841 gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct
     6901 gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa
     6961 gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac
     7021 caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt
     7081 attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca
     7141 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     7201 cggcagcgcc caacagtccc ccggccacgg ccctgccacc atacccacgc cgaaacaagc
     7261 gccctgcacc attatgttcc ggatcgtcaa tttcatagcc aagaagattt actgaaatat
     7321 acgcataccg cttacgtcaa agcgaaacaa atgaaattat tagttgatga tcagctttta
     7381 agtcttctac ttcagcaatc aataaaatat tttccaatag cttatcctaa tatgtgtgtg
     7441 acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta
     7501 gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata
     7561 agatttcata attgttccct gttccctgtt tatcacggaa gtgcaaaaaa caatataggg
     7621 attgataacc ttatagaagt gattacgaat aaattttatt catcaacaca tcgaggtcag
     7681 tctgaacttt gcggaaaagt tttcaaaatt gagtattcgg aaaaaagaca gcgtcttgca
     7741 tatatacgtc tttatagtgg tgtactgcat ttgcgagatt cggttagaat atcggaaaag
     7801 gaaaaaataa aaattacaga aatgtatact tcaataaatg gtgaattatg taaaatcgat
     7861 aaggcttatt ccggggaaat tgttattttg tagaatgagt ttttgaagtt aaatagtgtt
     7921 cttggagata caaagctatt gccacagaga gagagaattg aaatccccct ccctctgctg
     7981 caaacgactg ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta
     8041 gaaatctccg acagtgaccc gcttctgcga tattatgtgg attctgcgac acatgaaatc
     8101 atactttctt tcttagggaa agtacaaatg gaagtgactt gtgctctgct gcaagaaaag
     8161 tatcatgtgg agatagaaat aaaagagcct acagtcattt atatggaaag accgttaaaa
     8221 aaagcagagt ataccattca catcgaagtt ccaccgaatc ctttctgggc ttccattggt
     8281 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
     8341 ggatagttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggctgtgaa
     8401 caaggattgt atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac
     8461 tatagccctg ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa
     8521 gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg
     8581 ccacaggaat atctttcacg agcatacacc gatgctccta aatattgtgc gaacatcgta
     8641 gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt
     8701 caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag
     8761 ttaaaagggt accg
//
